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Research Associate Professor
of Pathology and Laboratory Medicine

Molecular Regulation of ApoB mRNA Editing
Phospholipid Scramblases

URMC Labs
601 Elmwood Ave., Rm G-2150
Rochester, NY 14642-8626
Tel: (585) 276-3014
Fax: (585) 276-2272

Mark_Sowden@urmc.rochester.edu

Qualifications

Ph.D., University of Oxford, England 1990
Postdoctoral Fellow, HPV Biology, University of Rochester, 1990-92
Postdoctoral Fellow, RNA Editing, University of Rochester, 1992-95

Research Overview

Dr. Sowden received the University of Rochester Deans Institutional Award in 2002-04 to study the role of activation induced deaminase as an RNA editing enzyme in human B-cell function. Since then, he has received support from the UR Environmental Health Sciences Center and the Philip Morris External Research Program for related studies of smoking-associated carcinogenesis and teh APOBEC family of deaminases.

He is currently working with Dr. Peter Sims and Dr. Therese Wiedmer in studies of phospholipid scramblases.

Publications

Lehmann DM, Galloway CA, MacElrevey C, Sowden MP, Wedekind JE, Smith HC. Functional characterization of APOBEC-1 complementation factor phosphorylation sites. Biochim Biophys Acta. 2007 Mar;1773(3):408-18. Epub 2006 Dec 8.

Bennett RP, Diner E, Sowden MP, Lees JA, Wedekind JE, Smith HC. APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic. Biochem Biophys Res Commun. 2006 Nov 10;350(1):214-9. Epub 2006 Sep 18.

Lehmann DM, Galloway CA, Sowden MP, Smith HC. Metabolic regulation of apoB mRNA editing is associated with phosphorylation of APOBEC-1 complementation factor. Nucleic Acids Res. 2006 Jul 4;34(11):3299-308. Print 2006.

Ichikawa HT, Sowden MP, Torelli AT, Bachl J, Huang P, Dance GS, Marr SH, Robert J, Wedekind JE, Smith HC, Bottaro A. Structural phylogenetic analysis of activation-induced deaminase function. J Immunol. 2006 Jul 1;177(1):355-61.

Sparks JD, Collins HL, Chirieac DV, Cianci J, Jokinen J, Sowden MP, Galloway CA, Sparks CE. Hepatic very-low-density lipoprotein and apolipoprotein B production are increased following in vivo induction of betaine-homocysteine S-methyltransferase. Biochem J. 2006 Apr 15;395(2):363-71.

Xie K, Sowden MP, Dance GS, Torelli AT, Smith HC, Wedekind JE. The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1.
Proc Natl Acad Sci U S A. 2004 May 25;101(21):8114-9. Epub 2004 May 17.

Smith HC, Bottaro A, Sowden MP, Wedekind JE. Activation induced deaminase: the importance of being specific. Trends Genet. 2004 Jun;20(6):224-7. Review. Erratum in: Trends Genet. 2005 Jan;21(1):36.

Sowden MP, Lehmann DM, Lin X, Smith CO, Smith HC. Identification of novel alternative splice variants of APOBEC-1 complementation factor with different capacities to support apolipoprotein B mRNA editing. J Biol Chem. 2004 Jan 2;279(1):197-206. Epub 2003 Oct 21.

Reeder JE, Sowden MP, Messing EM, Klover P, Villa-Moruzzi E, Ludlow JW. Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell line. Cancer Cell Int. 2003 Jul 23;3(1):12.

Wedekind JE, Dance GS, Sowden MP, Smith HC. Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business. Trends Genet. 2003 Apr;19(4):207-16. Review. Erratum in: Trends Genet. 2003 Jul;19(7):369.

Galloway CA, Sowden MP, Smith HC. Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phase. Biotechniques. 2003 Mar;34(3):524-6, 528, 530.

Sowden MP, Ballatori N, Jensen KL, Reed LH, Smith HC. The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factors. J Cell Sci. 2002 Mar 1;115(Pt 5):1027-39.

Dance GS, Sowden MP, Cartegni L, Cooper E, Krainer AR, Smith HC. Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing. J Biol Chem. 2002 Apr 12;277(15):12703-9. Epub 2002 Jan 28.

Giangreco A, Sowden MP, Mikityansky I, Smith HC. Ethanol stimulates apolipoprotein B mRNA editing in the absence of de novo RNA or protein synthesis. Biochem Biophys Res Commun. 2001 Dec 21;289(5):1162-7.

Sowden MP, Smith HC. Commitment of apolipoprotein B RNA to the splicing pathway regulates cytidine-to-uridine editing-site utilization. Biochem J. 2001 Nov 1;359(Pt 3):697-705.