Instruments - Analysis

URMC FLOW CORE GUIDELINES

The following guidelines apply to all URMC Flow Cytometry Core Instrurments. If you have questions about these guidelines, please contact Tim Bushnell.

• Before getting access to any core instrument, you will need to be trained in the proper use of the equipment. If you have not been trained, please contact Tim Bushnell to arrange training.
• If your run is taking longer than expected and will run into another users time, they have the right to bump you at the time their run is to start.
• If you anticipate needing to use the machine after hours, please make sure you understand fully how to change the fluidics and shut-down the instrument.
• Time for instruments will be billed based on the time reserved and/or time used, whichever is longer.
• Reservations cancelled less than 48 hours before scheduled time will be charged for the time reserved.
• Follow all posted cleaning guidelines.  If you have questions, ask the core personnel.
• If you leave the instrument a mess for the next user or fail to follow the posted cleaning guidelines, you will be charged a 1/2 hour fee for core personal to clean the instrument.
• In the event the instrument you are booked on is down, the core personnel will notify you as soon as possible, and will make every effort to reschedule your experiment as close to your initial time as possible. This may require the use of a different instrument. If you are uncomfortable running this instrument, the core will assist you and waive the $20/hour operator assist fee.
• Experiments will be deleted off the database at the end of each week. They will be deleted off the local computer at the end of the month.
• Exported FCS files will be deleted off the computer once a week. The Core will not back up this data. If you are missing FCS files, they can be recovered from archived experiments.
• Do not change the filter configuration without first discussing changes with Tim. Inappropriate filter configuration can lead to erroneous data. If the filters are changed for a particular experiment, they should be returned to the default configuration when you are finished. It is always advisable to check the filter configuration before you begin.
• Do not make custom instrument configurations of the LSR. If you think you need one, discuss your need with Tim.
• When designing poly-chromatic experiments, it is strongly encouraged to make an appointment with Tim Bushnell to review the experimental design to make sure that it can be run on the LSR-II.
• Bring the proper controls!

Fee Structure:

Time booked (via online calendar) or time used (whichever is longer)

Billed at $40/hour (billed to the minute)

+$20/hr operated assisted runs (coming soon)

FACSCanto II Configuration: 

488 Laser  || 633 Laser  || 405 Laser

488 LASER:

633 LASER

405 LASER

Fee Structure:

Time booked (via online calendar) or time used (whichever is longer)

Billed at $50/hour (billed to the minute)
+$20 for operater assisted runs (coming soon)

HTS Compatable

LSR-II Configuration: 

488 Laser  || 633 Laser  || 407 Laser || 532 Laser

488 LASER:

633 LASER

407 LASER

532 LASER

Fee Structure:

Time booked (via online calendar) or time used (whichever is longer)

Billed at $42/hour (billed to the minute)
+$20 for operater assisted runs (coming soon)

HTS Compatable LSR-II (2-9905)

Second instrument (also HTS compatible)- MRBX 1-11314 (12-color)

LSR-II Configuration: 

488 Laser  || 633 Laser  || 405 Laser (12 Color here)

488 LASER:

633 LASER

405 LASER

405 LASER (12 Color variant) The 12-color LSRII is located in the Flow Lab at 1-11314.