Rochester Oral Biology Research Conference

Abstracts

Pro-inflammatory Cytokines Alter Tight Junction Structure, Function in Par-C10 Cells, O.J. Baker, J.M. Camden, R.S. Redman*, C.I. Seye, L. Erb, and G.A. Weisman, Department of Biochemistry and Life Sciences Center of the University of Missouri, MO, USA, and *Veterans Affairs Medical Center, Washington, DC, USA.

The Role of Cftr in Submandibular Gland Ion Transport, Marcelo A. Catalán, Tetsuji Nakamoto, Victor G. Romanenko and James E. Melvin. From the Center for Oral Biology in the Aab Institute of Biomedical Sciences, University of Rochester Medical Center, Rochester, New York 14642

Rho Kinase signaling regulates cleft elongation during SMG Branching Morphogenesis, William P. Daley*, Katherine Gulfo, and Melinda Larsen. *Graduate Program in Molecular, Cellular, Developmental, and Neural Biology; Department of Biological Sciences, University at Albany, State University of New York, Albany, NY

Regulatory Pathway Changes During Parotid Acinar Cell De-differentiation, D.S. Darling, S.G. Venkatesh; Oral Health and Systemic Disease, PEDH, Univ. of Louisville School of Dentistry, Louisville, KY.

Analysis of salivary parameters from children with dental caries, in differents ages and gender.
Farghali JG, Leite MF, Fachin LV, Guaré RO. Universidade Cruzeiro do Sul. São Paulo, SP, Brazil.

Use of Yeast Mutant Collection to Study MUC7-12-mer Peptide Targets, J.R. Fuss, M. Lis, and L.A. Bobek, State University of New York at Buffalo, USA

Glycogen content of diabetic rats’ salivary glands after muscarinic stimulus, Emily Ganzerla*, Paula M. Yamaguti, Alyne Simões, José Nicolau, Oral Biology Research Center, Biomaterials and Oral Biochemistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.

Functional Equivalence between Osr1 and Osr2 in Mouse Development, Yang Gao, Yu Lan, Catherine E. Ovitt, Rulang Jiang, Department of Biomedical Genetics and Center for Oral Biology, University of Rochester, NY, USA

Mass Spectrometry Analysis of Affinity-Purified Parotid Plasma Membrane Proteins, Mireya Gonzalez-Begne*, Bingwen Lu, Lujian Liao, James E. Melvin, John R. Yates III , Center for Oral Biology, University of Rochester Medical Center; Rochester, New York, 14642,Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, SR-11, La Jolla, CA 92037

NBCe1 but not NBCn1 undergoes endocytosis in parotid acinar cells, Irina I. Grichtchenko, Daniel Freedman, David O. Quissell, Mary E. Reyland, Angelique Rocafort-Ward and Clint Perry, Department of Physiology and Biophysics, University of Colorado and Denver Health Sciences Center, Aurora, CO, USA 80045, Department of Craniofacial Biology, University of Colorado and Denver Health Sciences Center, Aurora, CO, USA 80045

Extracellular salt prevents cell wall binding and toxicity of Histatin, Woong Sik Jang *, Jianing N. Sun, Namrata Nayyar, and Mira Edgerton, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY

The role of Aquaporin 5 in salivary gland function. Jitesh D. Kawedia, John N. Lorenz, Gregory P. Boivin, Arthur R. Hand, Stephen Dewhurst, James E. Melvin and Anil G. Menon, Department of Molecular Genetics, Biochemistry and Microbiology, Department of Molecular and Cellular Physiology, Department of Pathology and Molecular Medicine, University of Cincinnati, School of Dental Medicine, University of Connecticut Health Center, Farmington, Center for Oral Biology, University of Rochester

Role of starch and sucrose in Streptococcus mutans biofilms formation, Marlise I. Klein, Simone Duarte, Hyun Koo, Eastman Department of Dentistry, University of Rochester, Rochester, NY, USA., Center for Oral Biology and Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA.

Epithelial-neuronal communication regulates embryonic salivary gland morphogenesis, Sarah M. Knox and Matthew P. Hoffman, Matrix and Morphogenesis Unit, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr, MSC 4370, Bethesda, MD 20892-4370, USA

Parotid Enlargement Associated with Diabetes Mellitus: Case Study and Review. E.M. Lai, MS, F. Younai, DDS. Department of Oral Medicine, UCLA Dental Center, Los Angeles

Glycogen concentration in salivary gland of streptozotocin-induced diabetic rats treated with sodium tungstate, Mariana Ferreira Leite, José Nicolau, Oral Biology Research Center of University of São Paulo, University Cruzeiro do Sul, São Paulo – Brazil

Interactions between antimicrobial peptide MUC7 12-mer and Candida albicans, Maciej Lis and Libuse A. Bobek, Department of Oral Biology, State University of New York at Buffalo.

Effects of changes in cell volume on ER-PM Ca2+ signaling, Xibao Liu, Hwei Ling Ong, Biman Paria and Indu Ambudkar, Secretory Physiology Section, Molecular Physiology and Therapeutics Branch, National Institutes of Dental and Craniofacial Research, Bethesda, MD, USA 20892.

Determination and Purification of Adult Mouse Submandibular Gland Stem Cells. Isabelle M.A. Lombaert, Jeanette F. Brunsting , Pieter K. Wierenga , Harm H. Kampinga , Gerald de Haan , Robert P. Coppes, Department of Cell Biology; section Radiation & Stress Cell Biology, Department of Cell Biology; section Stem Cell Biology, Department of Radiation Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands; National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, USA

Ssa1 proteins in cell adhesion and virulence of Candida albicans, N. Nayyar*, W. Li, N. Solis, S. G. Filler, A. Dongari-Bagtzoglou, and M. Edgerton (University at Buffalo, Buffalo, NY; Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, and University of Connecticut, Farmington, CT, USA)

Uncoupling Salivary Gland Dysfunction from Inflammation in a Large Cohort of Primary Sjögren’s Syndrome Patients. Nikolay P. Nikolov1, Ilias Alevizos1, Jane C. Atkinson2, Margaret Grisius1, Ana P. Cotrim3, Bruce J. Baum3, David Kleiner4, Jaime S. Brahim2, Lolita Bebris1, Rose Anne Leakan1, Gabor G. Illei1. 1 Sjögren’s Syndrome Clinic, NIDCR, NIH, Bethesda, MD; 2 Center for Clinical Research, NIDCR, NIH, Bethesda, MD; 3 Gene Transfer Section, NIDCR, NIH, Bethesda, MD;
4 NCI, NIH, Bethesda, MD

Adrenergic-stimulus and glycogen metabolism in salivary gland of diabetic rats, Fernando N. Nogueira*, Emily Ganzerla, Paula M. Yamaguti, Alyne Simões, José Nicolau, Oral Biology Research Center, Biomaterials and Oral Biochemistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.

Impaired trafficking and function of TRPC1 in Caveolin1 deficient mice, Biswaranjan Pani and Brij B. Singh, Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND - 58203.

Upregulation and requirement of TRPM7 in FGF2-dependent salivary gland growth and branching morphogenesis. Biman C. Paria, William Swaim, Vaishali Patel, Xibao Liu, Hwei Ling Ong, Katherine J. Chewning, Preeti Kansal, Matthew Hoffman, and Indu S. Ambudkar. Secretory Physiology Section, Molecular Physiology and Therapeutic Branch, and Matrix and Morphogenesis Unit, Cell and Developmental Biology Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.

Heparan sulfate 3-0-sulfotransferases regulate FGF10-mediated submandibular gland epithelial morphogenesis., Vaishali Patel*, Keyonica S. Lassiter, Samuel N. Cowherd, and Matthew P. Hoffman., Matrix and Morphogenesis Unit, CDBRB, NIDCR, NIH, DHHS, Bethesda, MD, USA.

Control of SMG epithelial cell differentiation by substrate rigidity, Sarah B. Peters*, Chen Wang*, Melinda Larsen. Molecular, Cellular, Developmental, and Neural Biology Program*, Department of Biological Sciences, University at Albany, State University of New York, Albany, NY.

Role of MT2-MMP during mouse submandibular gland morphogenesis, Ivan T. Rebustini, Christopher Myers, Keyonica Lessiter, Andrew Surmak, Ludmila Szabova, Kenn Holmbeck and Matthew P. Hoffman* Matrix and Morphogenesis Unit, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr, Bethesda, MD 20892, SA. Matrix Metalloproteinases Unit, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr, Bethesda, MD 20892, USA.

The Mechanism of Cholesterol-dependent Regulation of the IK1/maxi-K Interaction. Victor G. Romanenko1, 2, Kurt S. Roser 3, James E. Melvin1, 2 and Ted Begenisich1, 2.1The Department of Pharmacology and Physiology, the Center for Oral Biology2, and the Department of Anesthesiology3, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642

Gremlin and BMP7 in salivary glands exposed to hyperglycemia, Takashi Shinomiya, Mitsuru Kawaguchi, Department of Pharmacology, Tokyo Dental College

Solute Carrier- SLC5A1/SLC2A1 Gene Expression in Salivary Glands of Diabetic-Hypertensive Rats, Silva, RS; Alves-Wagner, ABT; Okamoto, MM; Alves, AS; Freitas, HS; Machado, UF. Department of Physiology and Biophysics of Institute of Biomedical Sciences, University of Sao Paulo

Mapping of Salivary Histatin 5 binding epitopes on C. albicans Ssa2p, Jianing N. Sun*, Namrata Nayyar, Woong Sik Jang and Mira Edgerton, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY.

Planar cell polarity in duct elongation during SMG morphogenesis,
Janice Walker, Sheede Khalil, A. Sue Menko and Maria A. Kukuruzinska, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania; and Department of Molecular and Cell Biology, Boston University School of Dental Medicine, Boston, Massachusetts

Proteomics-based Identification of Salivary Recognition Sites for Helicobacter pylori, A. WALZ, S. ODENBREIT, J. MAHDAVI, T. BORÉN, and S. RUHL, University of Regensburg, Germany, LMU München, Germany, Queen's Medical Centre, Nottingham, England, Umeå University, Sweden

Influence of dietary carbohydrates on Streptococcus mutans biofilm structure-architecture, Jin Xiao, Marlise I. Klein, Hyun Koo, Eastman Department of Dentistry, University of Rochester, Rochester, NY, USA, Center for Oral Biology and Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA, State Key Laboratory of Oral Diseases, Sichuan University, China.

Salivary glandular response to fluoride-induced oxidative stress and lipid peroxidation. Paula M. Yamaguti*, Emily Ganzerla, Fernando N. Nogueira, Douglas N. Souza, José Nicolau, Oral Biology Research Center, Biomaterials and Oral Biochemistry Department, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.